“How to Spit” A guide on how to provide a saliva sample for genetic testing

Instructions, tube, and packaging for a "spit kit" for DNA genetic testing
You’ve just received your very own saliva collection kit- congratulations!
You ’ ve barely received your very own saliva solicitation kit- congratulations ! now you ’ ra impart with the slightly daunt job of providing a sample. Set aside etiquette concerns and start thinking about your front-runner meal or snack- let ’ s get those saliva glands into entire gear !
Step 1: Nothing by mouth for 30 minutes
I hope you read all the instructions included in the kit beginning, but pause here if you ’ re not certain. We besides have this bang-up television. Please do not have anything by mouth for 30 minutes ( or more ) prior to collecting your saliva sample distribution .
nothing by mouth means nothing in or near your mouth that could provide another deoxyribonucleic acid sample distribution or dilute your own sample distribution. This means no eat, drink, smoking, chew, kissing, or toothbrushing.

We want to make sure it is only your sample and we have lots of your deoxyribonucleic acid to test. So this includes no water to drink or wet your mouth ; no hint mints, gingiva, or gargle to make surely your sample is “ fresh ” ; no kissing partners, children, or pets, etc- nothing !
very well to have : Chapsticks, lipsticks, breakfast an hour ago, ditching the gum at least 30 minutes earlier, taking pills at least 30 minutes before the sample collection, being pregnant, being brainsick, etc. You can have that nosh nearby to help you salivate, but wait to enjoy until after you ’ ve completed this task !

Step 2: Remove the kit and tube from the box
possibly not ampere fun as unboxing that newly earphone or other buy, but get your materials in front of you. It should include a cardboard corner with a fictile container holding the saliva tube. The tube has marks on the side to indicate the top and bottom of the saliva collection area, a top filled with a liquid that will help get your sample safely to the lab ( stabilize your deoxyribonucleic acid ), a corner, shipping envelope and shipping tag to get the kit back to the lab through FedEx or USPS .
Step 3: Label the tube
Take a cardsharp or other pen ( preferably non-washable and dark so it ’ s easily to read ) and put your full name and birth date on the tube. Some laboratories have nice bang-up labels on the tubes already. Others have a elementary barcode on the tube and it ’ s up to you to decide where to put your name. Please do your best to make it legible and to not cover any barcodes on the tube .
alternatively, you can use a label you have at home- write your name and give birth date on a blank label then attach to the tube or use an address label that you have and add your birthday-make indisputable it ’ s your name on the label not a rear, collaborator, spouse, or other family extremity. besides, please be sure to add the date of your sample distribution.

It ’ second amazing how many people forget this footprint. It ’ s partially the nerves of providing an wholly new sample type and partially the phlebotomist and other healthcare staff would normally handle this character for you at a rake draw. But all spit looks the same and we want to make certain they know it ’ s your sample distribution. besides, if the tube is not labeled, the lab will request a new sample, delaying your results. It helps to do this step earlier in the process, specially if multiple people in the house are providing samples .
Step 4: Provide the sample
immediately that you ’ ve done all the homework function, it ’ s time to provide the saliva. It is a little wyrd and awkward, to be indisputable, and it takes a batch of muscleman coordination to consciously spit in a minor space. There is a big mouthpiece on top of the collection tube. You must get the liquid part of the saliva over the black line, the bubbles don ’ thymine count. Some saliva is very bubbling while early saliva has about no bubbles. It takes most adults a few minutes to make or produce enough saliva to get the sample over the top line. For those people with dry mouths, it may take longer, then be affected role with yourself .
Some people find it helpful to wait a minute to collect saliva in their mouths to provide more saliva per spit. Others want to spit adenine soon as they make saliva so they don ’ metric ton forget and swallow. It ’ s up to you-there ’ randomness no right or improper manner to do this part–but most find it easier to hold a moment and get a larger amount of saliva quick before spitting .
Step 5: Close the tube and package the sample
once you ’ ve reached the magic level of spit, aka your liquid saliva is over the charming line, close the eyelid until you hear a click or pop. This is the hat close up wholly and releasing the stabilizing liquid that was in the top. Make certain all that liquid drips down into the metro, then unscrew the acme dowry. You can discard or throw away the lead hat. now remove the small hat from the little credit card container, it normally has a blue clear. Carefully screw on the modest hat, making certain to close it tightly. now shake or invert the tube a number of times to mix the saliva and liquid. This can be a similar motion to mixing your front-runner nail polish, mixing a swallow, child bottle, shake slant, or inverting randomness sample in high school chemistry .
Confirm that your name and birth go steady are on the tube. Place the tube back inside the plastic container or sample distribution bag. Place the container in the ship box. Place the ship box inside the ship envelope if included. Seal the envelope. Add the embark pronounce. Call the chain mail carrier to pick up the kit, or drop off at the nearest FedEx dribble box or office or USPS. ( Instructions may differ between different laboratories, so please read what is included with your kit out. Call us if you ’ rhenium uncertain. )
Tips and Tricks

If you find yourself having trouble making enough saliva sample these :

  • Gently massage your cheeks
  • Gently gnaw or chew on your tongue
  • Think about something sour like your favorite sour candy or lemons
  • Think about what you ate last or are going to eat next; imagine the smells, tastes and textures
  • Imagine you just finished brushing your teeth and need to spit out the toothpaste
  • If you’re really stuck, you can place a few granules of sugar on the tip of your tongue, just enough to get a taste of sweetness, and this will not affect the DNA sample.

There is no properly room to get saliva into the metro. There are ways that work good, but particularly children are very creative in their sample collection techniques. Go with what works for you vitamin a long as the saliva arrives in the tube .

  • You can hold the tube up to your lips and gently expel (spit) the saliva into the tube.
  • You can hold the tube just below your lips and let gravity help guide the saliva into the tube.
  • You can completely cover the tube with your lips (such as a small child drinking from a water bottle). Make sure to keep the tube vertical so the sample does not return home to your mouth.
  • You may be able to collect the sample all in one go, but this is rare. Most people cannot produce and hold that much saliva all at once. 
  • You can show younger children and adults having difficulty providing a sample how to do it by spitting into a nearby cup or sink. I’d encourage children to focus on the tube and explain that this is a special circumstance, and what the normal rules are about spitting, such as no spitting on people or furniture, or whatever your household rules are around this topic. 
  • In clinic I’ve noticed that most children are very eager to learn and have lots of questions about this process and what it means for after they leave clinic. Be honest and let us know if you come across questions that you need help answering.

Video production, filming, and editing credit : Maxine Stoll .

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